A New Rapid Real-Time PCR Method for Detection of Listeria monocytogenes Targeting the hlyA Gene
نویسندگان
چکیده
منابع مشابه
Rapid quantitative detection of Listeria monocytogenes in meat products by real-time PCR.
We describe a quick and simple method for the quantitative detection of Listeria monocytogenes in meat products. This method is based on filtration, Chelex-100-based DNA purification, and real-time PCR. It can detect as few as 100 CFU/g and quantify as few as 1,000 CFU/g, with excellent accuracy compared to that of the plate count method. Therefore, it is a promising alternative for the detecti...
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A rapid method for the detection of Listeria monocytogenes in foods combining culture enrichment and real-time PCR was compared to the ISO 11290-1 standard method. The culture enrichment component of the rapid method is based on the ISO standard and includes 24h incubation in half-Fraser broth, 4h incubation in Fraser broth followed by DNA extraction and real-time PCR detection of the ssrA gene...
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ژورنال
عنوان ژورنال: Food Science and Technology Research
سال: 2012
ISSN: 1344-6606,1881-3984
DOI: 10.3136/fstr.18.47